Purification and characterization of the cholinergic receptor protein in its membrane-bound and detergent-soluble forms from the electric organ of Torpedo marmorata.
نویسندگان
چکیده
De Robertis, E. (1976) in The Structural Basis of Membrane Function (Hatefi, Y . & DjavadiOhaniance, L., eds.), pp. 339-361, Academic Press, New York, San Francisco and London Harms, A. F. & Nauta, W. Th. (1960) J. Med. Pharm. Chem. 2,57-77 Jacobs, S . & Cuatrecasas, P. (1976) Biochim. Biophys. Acta 433,482-495 Kachadorian, W. A., Wade, J. B. & DiScala, V. A. (1975) Science 190, 67-69 Kasai, M. & Changeux, J. P. (1971) J. Membr. Biol. 6, 24-32 Maddy, A. H. & Dunn, M. J. (1976) in Biochemical Analysis of Membranes (Maddy, A. H., ed.),
منابع مشابه
A morphological study of the cholinergic receptor protein from Torpedo marmorata in its membrane environment and in its detergent-extracted purified form.
The highly specialized structural features of the postsynaptic membrane of Torpedo marmorata electric organ have been studied by freeze-fracturing, freeze-etching and negative staining applied both to intact electroplaques and to isolated purified excitable membrane fragments. The external surface of the postsynaptic membrane, revealed by deep-etching and negative staining, is characterized by ...
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The presynaptic plasma membrane (PSPM) of cholinergic nerve terminals was purified from Torpedo electric organ using a large-scale procedure. Up to 500 g of frozen electric organ were fractioned in a single run, leading to the isolation of >100 mg of PSPM proteins. The purity of the fraction is similar to that of the synaptosomal plasma membrane obtained after subfractionation of Torpedo synapt...
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The binding of a fluorescent agonist to the acetycholine receptor from Torpedo electric organ has been studied by time-resolved spectroscopy in three different environments: in native membrane fragments, in the detergent CHAPS, and after complexation by amphipathic polymers ('amphipols'). Binding kinetics was similar in the membrane and in amphipols, demonstrating that the receptor can display ...
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After fixation with glutaraldehyde and impregnation with tannic acid, the membrane that underlies the nerve terminals in Torpedo marmorata electroplaque presents a typical asymmetric triple-layered structure with an unusual thickness; in addition, it is coated with electron-dense material on its inner, cytoplasmic face. Filamentous structures are frequently found attached to these "subsynaptic ...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 5 2 شماره
صفحات -
تاریخ انتشار 1977